Lyme Disease Diagnostics: Culturing and Staining Procedures Essay

The case addresses a 27-year-old white girl introduced at her native doctor’s strolling clinic on August 15. She complained a few extreme headache and backache, lack of ability to pay attention, tender joints, fatigue, and a stiff neck. On bodily examination, the affected person’s fever was 38.5C, and the doctor moreover famous an irregular heartbeat and a round “rash” with a diameter of roughly 5 inches, vibrant pink forefront, and a dim centre in a “bull’s eye’s” type. According to the affected person, she has spent three weeks within the subject in Wisconsin throughout May and June monitoring small mammals as a graduate scholar inside a framework of the college’s wildlife program. As spring was moist and heat, a lady talked about a lot of mosquitoes, ticks, and biting flies within the space. Initially, she felt properly; nonetheless, after two weeks since her return, the primary signs appeared. As lots of them had progressed, the sufferers couldn’t tolerate her worsening situation anymore.

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On the idea of the affected person’s complaints, historical past, and bodily examination, it’s attainable to conclude that probably the most acceptable prognosis is Lyme illness. It is a multistage and multisystem bacterial an infection that could be brought on by a number of species of tick-borne spirochetes, “flexible, spiral-shaped bacteria that have a unique, internal flagellar arrangement,” within the household of Borreliaceae (Willey et al., 2020, p. 41). First of all, the reservoirs of this an infection in nature are birds and small mammals, and a human could purchase it from an contaminated tick’s chunk. Thus, spending a number of weeks within the territory with small mammals and a lot of ticks will increase the danger of the contraction of Lyme illness. In addition, in response to the Wisconsin Department of Health Services (2020), “Lyme disease is the most commonly reported illness spread by ticks in Wisconsin” (para. 2). It is historically unfold by contaminated immature ticks, referred to as nymphs, introduced within the state’s all counties. As nymphs are smaller as compared with grownup ticks, and their bites are painless, it’s harder to see them and take away them promptly with a purpose to stop an infection.

Subsequently, the affected person’s signs virtually absolutely correspond to the most typical signs of Lyme illness that impacts a number of organs and tissues, together with joints, coronary heart, pores and skin, and nervous system. An early-stage an infection that sometimes happens a number of weeks after a chunk is characterised by a selected pores and skin rash referred to as major erythema migrans (Bergström & Normark, 2018). A excessive focus of spirochetes within the blood causes fever and associated fatigue, and, untreated, and the illness could result in extra critical pores and skin problems, neurological issues, a stiff neck related to meningitis, myocarditis, everlasting arthritis, and broken joints (Bergström & Normark, 2018). In normal, Lyme illness has a number of distinctive virulence components, together with outer floor molecules that assist in “attachment, transmission and immunological escape of the pathogen” and supply interplay with the mammalian host (Bergström & Normark, 2018, p. 486). Moreover, spirochete’s outer membrane proteins contribute to its survival in a number, virulence, and transmission (Willey et al., 2020).

The diagnostics of Lyme illness implies a two-step method and goals to detect antibodies to spirochetes, which manufacturing is stimulated by micro organism’s proteins. Initially, the enzyme-linked immunosorbent assay (ELISA) take a look at ought to be made, and if its outcomes are optimistic, the Western blot take a look at is crucial with a purpose to affirm an infection. In laboratory settings, the Gram stain that means micro organism’s division into gram-positive and gram-negative is probably the most environment friendly and regularly used method for spirochetes’ direct detection (Willey et al., 2020). The remedy of Lyme illness historically contains antibiotics that assure a fast restoration within the majority of instances. Oral antibiotics, reminiscent of doxycycline, cefuroxime, and amoxicillin, could also be considered a regular remedy, particularly at early levels. However, if the an infection has already affected a affected person’s nervous system, intravenous antibiotics are required.


Bergström, S., & Normark, J. (2018). Microbiological options distinguishing Lyme illness and relapsing fever spirochetes. Wiener klinische Wochenschrift, 130, 484–490. Web.

Willey, J. M., Sandman, Okay. M., & Wood, D. (2020). Prescott’s microbiology (11th ed.). McGraw-Hill Education.

Wisconsin Department of Health Services. (2020). About Lyme Disease. Web.

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